|
KMID : 0357319930280040295
|
|
Journal of the Korean Society for Microbiology
1993 Volume.28 No. 4 p.295 ~ p.302
|
|
|
Comparison of Capillary Transfer, Vacuum Transfer and Electro-transfer in DNA Analysis
|
|
서민호
황미열/백성덕/백원기/서성일/박종욱/김재룡
|
|
|
Abstract
|
|
|
In order to evaluate the efficiencies of various DNA blotting methods for routine clinical laboratory application of Southern blotting, we compared three blotting methods, i.e. capillary, vacuum, and electric. We used serially diluted
concentrations of
HindⅢ-digested lambda DNA for large molecular ranges(23.130-2.027 Kb), and 123 DNA ladder for small molecular ranges(0.123-4.182 Kb). In capillary blotting, the efficiencies of DNA transfer both in large and small molecular ranges were good, but
it
took 18 hours to transfer DNA of 23 Kb in size. And the procedures were somewhat labor-intensive. In electric blotting, the efficiency of DNA transfer was good and blotting time was relatively short (2 hours). But the amount of blotting buffer
consumption was as many as 5,000ml and the cost of electric transfer apparatus was high. In vacuum blotting, the efficiencies of DNA transfer both in large and small molecular ranger were good, and blotting time was very short(only 1 hour). The
amount
of blotting buffer consumption was only 70ml, and handling was very simple in vacuum blotting procedure.
In conclusion, the vacuum blotting method is very rapid, simple, efficient and economic ; so we think this method is the best choice for routine clinical laboratory application of Southern blotting.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|